It depends on how the timing controller communicates with the MCU. If the timing controller is powered by Li+ it has the possibility of being 3.6V to 4.2V, which has logic levels higher than the nRF52 in the Tracker SoM can handle.
If the timing controller is a receive-only device, you’re fine. If it’s an I2C device that can operate at 3.3V I2C you’re also fine.
However, if it uses SPI you need to level translate MISO as the signal from the timing controller will be at too high of a voltage for the Tracker SoM (or any Gen 3 device).
Likewise, serial to MCU would be an issue. Any interrupt pins that are push-pull (not open collector) would also need to be level-shifted.